Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. 1. Capture Flash Assays with Ease. ValitaTiter Plus settingsContact our support team with your questions regarding Molecular Devices products and applications. Published Dec 16, 2020 | Updated Dec 16, 2020. D 1 1. The calculated LOQ values were 0. . Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. Modularity does provide some measure of forward vision into upcoming or future experimental needs. MitoXpress-Xtra Oxygen Consumption Assay - Agilent. compound concentration, and an IC 50 value of 21. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. . SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. Bulk Kit. ai; ImageXpress Pico; IN Carta Image Analysis Software; CLONE SCREENING. The corrected absorbance is within 5% of the. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm pathlength cuvette. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel detection capabilities without the need for service. Figure 2-1 Label on Rear of Instrument WARNING!We would like to show you a description here but the site won’t allow us. SpectraMax® Microplate Readers and SoftMax® Pro Software. ZERO BIAS - scores, article reviews, protocol conditions and more. Adding time resolved fluorescence, HTRF® or western blot detection simply requires the user to exchange a detection module. to View Figures. Amplitude : 1. The SpectraMax® Paradigm Multi- Mode Microplate Reader with Tunable Wavelength (TUNE) Detection Cartridge from Molecular Devices combines the flexibility of wavelength scanning with the high sensitivity of filters. iD5 reader (the SpectraMax iD3 reader is also suitable for these assays, as it has the same onboard fluorescence performance as the SpectraMax iD5 reader), SpectraMax i3x, SpectraMax M4 or M5, and FilterMax F5 Multi-Mode Microplate Readers. ZERO BIAS - scores, article reviews, protocol conditions and moreThe SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. Price from $9. . In the second assay, HepG2 cells were plated into a 96-well clear-bottom, blackwalled plate at 10,000 cells/well and incubated overnight at 37°C. china@moldev. Wokingham, Berkshire RG41 5TS United Kingdom. Both readers demonstrated their ability to detect the HTRF signal from the terbium donor and red acceptor combination, and they could be used for saturation studies using the Tag-lite technology. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. . . Page 28 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 4. 030 fmol. 6% for the on-board optics (monochromators). As an aspirant Bioscience research with expertise in cell biology, immunology and bio instrumentation techniques, I bring a wealth of knowledge and expertise to the table. Homogeneous and eterogeneous microplate assays can be performed in one flexible system. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataSpectraMax i3x Multi-Mode Microplate Reader. Filterless readers provide consistent results with steady temperature regulation. 2. The spectra analysis and K d were determined using Sigmaplot 14. The SpectraMax i3x Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. Products : SpectraMax i3x reader, SoftMax Pro GxP Software, SpectraMax i3, SoftMax Pro Software, SpectraMax MiniMax cytometer When I attempt a read with my SpectraMax i3 or i3x reader using the injector cartridge, why do I see a plate height error, even though I have entered the correct plate height?. The information below applies to a standard 96 well plate, set to a linear shake mode. Microplate and read height should be optimized for each new lot of microplates or assay volume used (use the Show Pre-Read Optimization options in the instrument settings in SoftMax Pro Software). AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. Analysis. The M5 can read volumes down to 2uL with the uMax Low. Molecular Devices社の製品: 吸収度マルチモードマイクロプレートリーダー SpectraMax® i3x に関するすべての情報をご覧ください。. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. . 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideDiscover how the automation-friendly SpectraMax® i3x Multi-Mode Microplate Reader, designed to expand research capabilities through its versatile and user-upgradeable application modules, can. SpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. The SpectraMax DuoLuc Reporter Assay has been performance optimized on the SpectraMax i3x reader with Injector Cartridge. 302 low ref light. Results for SpectraMax i3x reader with NanoBRET Detection Module (blue) and on-board luminescence detection mode (orange). The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 0´ 105 dsD NA (p g/ wel ) R F UFluorescence intensity was measured using a Spectramax i3x plate reader (Molecular Devices) with excitation at 575 nm (bandwidth 9 nm) and emission at 608 nm (bandwidth 15 nm). Antigen Quantitation Protocol. The heating element design of the category 1 microplate readers, such as the SpectraMax i3x Multi-Mode Microplate Reader, applies a dual area sensor for temperature control. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideProducts : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. If no sound is heard during initialization, contact technical support. Pancreatic lipase activity was determined by measuring the absorbance of the test solution at 405 nm with a micro plate reader (MD SpectraMax i3x). The chamber. 2. SpectraMax i3x Multi-Mode Detection Platform User Guide. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Eliminate time-dependent substrate addition steps. . Products : SoftMax Pro Software, SoftMax Pro GxP Software, CellReporterXpress software, General, Screenworks, pCLAMP/AxoScope, GenePix Pro, MetaXpress software. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. SpectraMax Microplate Reader - Choose from our extensive SpectraMax® line of user-friendly microplate readers. i3x. Multimode Reader Comparison Chart. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Assay setup for a 384-well low-volume plate. It allows you to explore cellular pathways and protein activation and expression in one system. The Relative Luminescence Units (RLU) were measured using the SpectraMax i3x Luminescence Glow cartridge (Lum 384) (0200-7015POS, Molecular Devices) and normalized to the cell count or area, which were determined using the Mini MaxTM 300 Imaging Cytometer (5024062, Molecular Devices). application modules • Multi-mode microplate reader and imaging system • Wide dynamic range • StainFree™ cell counting technology • Library of user-configurable applications • Advanced curve fitting and statistical analysis • Cell confluence and cell viabilitySoftMax® Pro 7. VAL014, right table) assays on the SpectraMax i3x reader are shown below. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. Enzyme Activation Analysis;. SpectraDrop Abs Protein Quant (i3-i3x)Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Quantification of NETs was performed with the SpectraMax i3x microplate-reader (Molecular Devices, United Kingdom). The SpectraMax Injector Cartridge enables precise reagent delivery and adjustment of delay and integration times that are required to obtain optimal sensitivity for this assay. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The SpectraMax i3x Multi-Mode Detection Platform User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax i3x, a versatile and customizable microplate reader that can perform various assays and imaging tasks. Explore a wealth of applications with the future-ready SpectraMax i3x. Dilution series of HeLa cells transfected with firefly (red plot) and Renilla (blue plot) luciferases. SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. 2 Note Section Patch:1) Select the correct plate and the wells that you want to measure. . Rutin was used to generate standard curves for quantification. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Average 98 stars, based on 1 article reviews. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. The treatments were. Microplate Readers SpectraMax. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. The TRF intensities measured are proportional to the level of extracellular acidification in each sample. The plate was covered and incubated for one hour at room temperature. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologyMeasure oxidative metabolism and glycolytic activity on the SpectraMax i3x reader. 313 reference gain check fail. using the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. No accessories are needed and SoftMax® Pro. Example: [Setup]. Introduction. Controls containing all reaction. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. 1. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex. Order Now. Materials and methodsSpectraMax i3x Multi-Mode Microplate Reader. techsupport. Western blot assay 5 x 105 cells were seeded in 24-well plates in 500 μl growth medium and stimulated with novel compounds at 10μM. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. +1 800-635-5577. 3 Figures from 612 Publications Promega. This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. Cet appareil ISM est confomre à la norme NMB-001 du Canada. Structured Review. user upgradeable. Figure 4. 6% for the on-board optics (monochromators). With optimized reagents, option to operate the. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. Measured using the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. . 1. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. The SpectraMax i3x Multi-Mode Microplate Reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. eu@moldev. ZERO BIAS - scores, article reviews, protocol conditions and more. The MiniMax cytometer with SoftMax® Pro Software can be used to image and analyze a variety of cells. Bioz Stars score: 98/100, based on 1 PubMed citations. SpectraMax® i3 Multi-ModeDetectionPlatform withtheSpectraMax® MiniMax™ImagingCytometer UserGuide 5025027A April2013. 7 Figures from 1126 Publications. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. As per Volza’s India Import data, Spectramax import shipments in India stood at 365, imported by 145 India Importers from 28 Suppliers. SoftMax Pro Software enables users to quickly set up analysis of cell counts via a simple point and click user interface. 3. . Supplier: Molecular Devices. Published Dec 16, 2020 | Updated Apr 13, 2023. ü; SpectraMax® Mini reader support. SpectraMax i3x, onboard luminescence 121. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. It allows you to explore cellular . Price from $9. For wavelengths in the UV range above 220 nm,. Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. *On the SpectraMax i3x reader only. Database contains 1 Molecular Devices SpectraMax i3x Manuals (available for free online viewing or downloading in PDF): Installation manual . SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析We would like to show you a description here but the site won’t allow us. Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays, enzyme. 2 Parallel Line Analysis Conc log [ng/ML] RLU. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). 312 gain calibration failed. The SoftMax® Touch Software is installed in. Eight different automated tests are provided: • Absorbance accuracy (linearity) • Precision (reproducibility) • Stray light • Wavelength accuracy • Wavelength repeatability SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Genscript cPass SARS-CoV-2 Neutralization Antibody Detection. MC38 in vivo tumor model in hPD-1. Products : SoftMax Pro GxP Software, SoftMax Pro Software. Explore a wealth of applications with the future-ready SpectraMax i3x. . i3x single use: $35: $52. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. Automated for ease of use. Monday to Friday 7am - 5pm Pacific Time. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with. Actual shake speed is based on the microplate format. HTRF-compatible SpectraMax Multi-Mode Readers. Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. Biochrom New EX Read 400 Microplate Reader. . . The user guide also covers the features and specifications of the SpectraMax MiniMax 300 Imaging Cytometer, an optional accessory that. ü; Instrument Diagnostics Mode (for SpectraMax® i3x, SpectraMax® iD3, SpectraMax® iD5, SpectraMax® Paradigm)Absorbance was measured on the SpectraMax i3x Multi-Mode Platform at 475 nm and 660 nm after 1-4 hours of incubation. SpectraMax® i3x Multi-Mode Detection Platform, Molecular Devices. Multimode Readers Comparison Chart. Asterisks indicate the dilution of assay control that should be minimally detectable for each kit. The microplate was then placed in a SpectraMax i3x reader and incubated at 37°C for the duration of the kinetic measurement. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 99. The dynamic range of the assay is about 3. 1. . SpectraMax i3x Register or Sign In. User-upgradeable detection modules allow you to expand the capabilities of your microp. user upgradeable. FTIR Spectrometer: Shimadzu IRSpirit (2018)The plate was read on the SpectraMax i3x Multi-Mode Microplate Reader. 22mm 384 FilterMax™F3&F5Multi-ModeMicroplateReaders,SpectraMax®i3x Multi-ModeDetectionPlatform,SpectraMax®iD3&iD5Multi-Mode MicroplateReaders,andSpectraMax®Paradigm®Multi-Mode MicroplateReader 5301193 18mm 96 SpectraMaxi3x,SpectraMaxiD3,SpectraMaxiD5,SpectraMaxM. View the Molecular Devices website. ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析 We would like to show you a description here but the site won’t allow us. TR-FRET ratios were calculated by dividing the RFU value for the 665-nm reading by the RFU value for the 616-nm reading, and the resulting numbers were multiplied by 1000. Transcreener® HTS is a universal, high throughput biochemical assay platform based on the detection of nucleotides, which are formed by thousands of cellular enzymes—many of which catalyze the covalent regulatory. SoftMax Pro User’s Manual v Contents Figures. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. We would like to show you a description here but the site won’t allow us. SpectraMax i3/i3x Multi-Mode Microplate Reader SoftMax Pro Software 0 500 1000 1500 2000 2500 3000 0 1´ 106 2´ 106 3´ 106 dsDNA (pg/we l) R F U 02 4 6 8 1 0 2. The user guide also covers the features and specifications of the SpectraMax MiniMax 300. Unplug the power cable from the back of the instrument. . 13 There are a fairly limited number ofSARS-CoV-2 variants of concern (VOCs) that increase transmission or disease severity or reduce diagnostic or vaccine efficacy continue to emerge across the world. Catalog excerpts. Finally, their fluorescence was measured with 365-nm excitation and 450-nm emission filters using a microplate fluorometer (SpectraMax i3x, Molecular Devices, San Jose, CA, USA) (Saiya-Cork et al. Average 98 stars, based on 1 article reviews. SpectraMax i3x Multi-Mode Microplate Reader. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast. 0´ 104 6. Quantiferon-TB Gold in Tube Kit. 2. Product Details. Find regional support. SpectraMax iD3 reader with touchscreen and inserted USB flash drive. ZERO BIAS - scores, article reviews, protocol conditions and moreThe SpectraMax ® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and. Superior flexibility The SpectraMax i3 Multi-Mode Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. StackMax Microplate Handling System. Real-time measurement of extracellular oxygen depletion and acidification in cultured cells and microorganisms. each new lot of plates, to ensure that the center of each well is read. . Ease of use. pH-Xtra Glycolysis Assay - Agilent. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. . . Monochromator optics with adjustable bandwidth offer excellent sensitivity down to 6 nM tryptophan, as well as precise determination of the fluorescence emission peak shift that occurs upon protein denaturation. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when. SpectraMax Paradigm Platform in conjunction with AlphaLISA provides a powerful tool to screen novel therapeutics in a pathologically relevant, endothelial cell-based model. Microplate optimization should be performed the first time the assay is run, and subsequently for . Microplate readers, multi-mode, SpectraMax® i3x. 0´ 104 1. Examples: “Press carefully to engage,” “ Do not. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. Sensitive TRF detection of two simple, mix-and-measure assay workflows. Read more about HTRF SpectraMax Detection Cartridges for SpectraMax i3x & Paradigm Systems which utilize a high energy Xenon flash lamp for the sensitive reading of HTRF reagents. 5577 Web: Email: info@moldev. . The SpectraMax i3x reader provides the. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for. Description 1. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. Flash-type luminescence with excellent sensitivity Expression measurement in as few as ten cells per well Improved accuracy of results by. . 因为是导师第一任学生，没有师兄师姐，加上本人悟性也差，小小pcr花了半年时间才终于有点样子。因为经历过，所以懂得，故而分享之，希望能给到屏幕前的你一点点帮助! 如有失误，欢迎指正~最后，不要忘了一键三联哟，谢谢大家！, 视频播放量 6293、弹幕量 2、点赞数 122、投硬币枚数 55、收藏. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. 4. User Manuals, Guides and Specifications for your Molecular Devices SpectraMax i3x Measuring Instruments. SpectraMax i3/i3x readers must be equipped with a suitableThe SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. 1、卡盒式模块和细胞成像模块的构架检测功能（如FP、TRF、HTRF、AlphaScreen、ScanLater Western Blot等）卡盒模块化设计，仅通过插入卡盒就能可. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. The SpectraMax® i3 Multi-Mode Microplate Reader Detection Platform evolves with your future research needs and offers a breadth of application possibilities. 003 fmol 0. SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. Facilities. Find regional support. AlphaScreen assays are compatible with our SpectraMax i3x, SpectraMax i3, and SpectraMax Paradigm Multi-Mode Microplate readers with the Alphascreen Detection Cartridge options. ZERO BIAS - scores, article reviews, protocol conditions and moreStructured Review. It has a cooled PMT, a no-stain cell analysis algorithm, and a field-upgradeable imaging cytometer. 020 fmol 0. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. BMG LABTECH VANTAstar. Image analysis provides accurate data. User-installable and removable detection cartridges allow the detector to be configured for specific applications and easily. (SpectraMax i3x and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most published microplate reader control and microplate data analysis software package available to scientists. Using the SoftMax Pro Workflow Editor, a kinetic cycle containing an absorbance read (PlateOD600) and a fluorescence read (PlateGFPBottom) with a 5-second linear plate shake between reads was created (Figure 1). Figure 3. 세포신호전달은 세포가 환경에 반응하고 다른 세포와 소통할 수 있게 합니다…. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. Data were analyzed with SoftMax ® Pro Software and graphed using a 4-parameter curve fit. EMax Plus Microplate Reader. com. Each heating element is independently. 01 0. GM2000 Register or Sign In. In brief, a pre-titrated dose of virus was incubated with 8 serial 5-fold dilutions of serum samples in duplicate in. The dual auto injector module. 99 to $1999. 7 Figures from 1126 Publications Promega. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate. . Both Microplate Optimization and Read Height Adjustment were. 6% for the on-board optics (monochromators). Prepare your i3/i3x instrument for shipment. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Background noise is reduced by the reader’s cooled PMT, enabling increased sensitivity and dynamic range. For each assay kit, a preconfigured protocol is included in SoftMax Pro Software to facilitate the acquisition and analysis of data for a simplified workflow. Version 7. Introduction Inflammation, the body’s reaction to infection, irritation, or other injury, is accompanied by increased endothelial chemokine. Pick the best speed, sensitivity, and price for your needs. 7. Engineered for high performance, the auto dual injector cartridge includes fail safe features. 0 mm hex key. Transfected cells were imaged on the SpectraMax MiniMax 300 Imaging Cytometer. Reporter Assay. The SpectraMax MiniMax 300 Imaging Cytometer option is a field-upgradeable option for the SpectraMax i3/i3x Multi-Mode Detection Platform that offers cellular imaging capability. Ease of use Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. Unlimited breadth of. Find regional support. Nucleic Acid and Protein Quantitation. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. 吸収度マイクロプレートリーダー SpectraMax® Mini. 303 unable to cal dark current. For more detailed information and technical support of Molecular Devices instruments or software,The SpectraMax i3x Multi-Mode microplatereader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging and Fast Kinetics with Injectors. ATP LLD (pM) ATP LLD (moles) SpectraMax i3x, luminescence . SpectraMax iD5:SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Description 1. Both readers feature an 8-channel read head for fast reads and. The SpectraMax i3x reader grows with you as you continue to advance your research, enabling you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 価格、見積もり、お近くの販売店を. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. The SpectraMax Paradigm Multi-Mode Detection Platform is a modular multi-mode microplate detector. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Wait 20 seconds. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. HTRF IP standard curve. . The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The calculated LOQ values were 0. This update is applicable for users of the SpectraMax® i3x Multi-Mode Detection Platform and SpectraMax® Paradigm® Multi-Mode Microplate Reader with the ScanLater Western Blot (WB) Detection Cartridge and the SpectraMax® iD5 Multi-Mode Microplate Reader: SMP 7. Register to download your free eBook today. The SpectraMax® i3x Multi-Mode Microplate Reader with the SpectraMax® MiniMax™ 300 Imaging Cytometer is equipped with transmitted light (TL), green fluorescence (Ex/Em: 460/541), and red (625/713) fluorescence channels. ，相关视频：多功能酶标仪SpectraMax i3x的维护操作-注射器卡盒，多功能酶标仪SpectraMax iD5的维护操作-注射器卡盒，多功能酶标仪SpectraMax i3x的维护操作-卡盒的安装和拆卸，细胞计数及活率分析仪 Vi-CELL BLU 功能&操作讲解，CRISPR基因编辑系统介绍：Cas9和Cas12a，流式. Citation 2002). 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. Prime function 20 amol. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Then, cells were washed with PBS three times and 100 μL of Nano-Glo HiBiT Lytic Reagent was added to. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeSpectraMax i3x and iD5 readers can be equipped with an HTRF detection cartridge or Enhanced TRF module and filters, respectively. Find regional support. 0´ 104 4. com Check our website for a current listing of worldwide distributors. . Price from $9. Controls containing all reaction. The intensity of the emitted light can be measured easily with the SpectraMax i3x reader. Download PDF. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Danaher Inc spectramax i3x. The SpectraMax i3x reader provides theSoftMaxProSoftwareFormulaReferenceGuide 8 5049093A SummaryFormulas Summariesconsistoffourparts: ASummaryname(whichisusedtorefertotheSummaryinotherformulas). 305 reference level saturation. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. 1. g. Other technologies such as imaging. TP medium was used as a blank and its fluorescence intensity was subtracted from that of mCherry or from chlorophyll auto-fluorescence intensity. 99. Concentrating the excitation light on the sample through label specific optics, high levels of sensitivity and performance are reached. SpectraMax® ABS and ABS Plus reader support; ü. . Multimode Reader Comparison Chart. 5 µL anti-cAMP-Cryptate. We would like to show you a description here but the site won’t allow us. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email drugdiscoverytech@lifetech. Note: Additional readers with similar performance are listed. were performed on a Biomek i7 containing an integrated orbital plate shaker and a SpectraMax i3x plate reader (Molecular Devices). Keywords: filtermax multi mode microplate readers, spectramax paradigm multimode detection platform, spectramax paradigm system Created Date: 11/16/2010 12:21:51 PMThe SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. Settings not required by a reader are indicated by ‘---’. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Cell viability는 세포막 Integrity 또는. Description. This process should take about 3 - 5 minutes. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. The SpectraMax ® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. 58% for the detection module and 2. User-upgradeable detection modules allow you to expand the capabilities of your microp. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Learn more about its features, specifications, and applications in this user guide. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Explore PDF for more details. Not compatible with Home Edition. SpectraMax i3x Multi-Mode Microplate Reader.